|
KMID : 1004820150160020060
|
|
Journal of Biomedical Research
2015 Volume.16 No. 2 p.60 ~ p.66
|
|
|
Chemopreventive effect of α-viniferin in azoxymethane-induced mouse colorectal tumor and Caco-2 cells
|
|
Kwak Dong-Hoon
Shin Sang-Kyung
Youm So-Young
Kim Tae-Wang
Kim Young-Soo
Ahn Byeong-Woo
|
|
|
Abstract
|
|
|
|
α-Viniferin (AVF), a trimer of resveratrol, is known to have an anti-inflammatory effect via inhibition of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). It has been reported that up-regulated COX-2 and iNOS are expressed in colon cancer tissues of humans and rodents as well as pre-neoplastic aberrant crypt foci (ACF) of rodents. In this study, chemopreventive effects of AVF were assessed in Caco-2 cells as well as azoxymethane (AOM)-induced colorectal tumorigenesis in mice. Anti-tumor effect of AVF with regards to apoptotic induction was assessed by TUNEL and caspase-3 expression in human colon cancer Caco-2 cells. For development of ACF, AOM was administered with to mice intraperitoneally at a dose of 10 mg/kg once a week for 3 weeks. To induce colitis-related colon cancer, mice were administered a single dose of AOM (10 mg/kg) and 2% dextran sodium sulfate in drinking water. Mice treated with 0.05 and/or 0.1 mg of AVF by gavage showed significantly reduced development of ACF and colorectal tumors. Immunofluorescence detection in Caco-2 cells showed reduced COX-2 and iNOS expression, whereas cleavage of caspase-3 and apoptotic cell numbers increased upon AVF treatment. Immunostaining showed reduced expression levels of COX-2 and iNOS expression along with increased cleaved caspase-3 expression increased upon AVF treatment. These results suggest that AVF has chemopreventive effects on colorectal cancer via anti-inflammatory potential and pro-apoptotic activity.
|
|
|
KEYWORD
|
|
|
α-viniferin, Caco-2 cell, chemoprevention, colorectal cancer, mouse
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|